The couple of tries I've made thus far have worked really well. Now, if you select a bunch of gates (shift-click them, or cmd+a to select all of them) you can hit cmd+opt+m to 'Mold' the gates together. This gets back to the point of not being able to get all my polygons to match up exactly with no whitespace and no overlap.
The second new gating function is what they call molded gates. A small price to pay, I think, but hey guys, if you're listening, that'd be nice. You need to do a select all gates function (cmd+a) and then grab the center. Wonderful! My only complaint in actually using this is that after you convert your standard quad gates into a spider gate, you can no longer simply grab the center point and move all 4 quads at the same time. Just option-click the center point of the quads and move to bend the quad lines.
But now, FlowJo has introduced in the Mac version 8.8 (go figure, the Windows version falls behind again) 'Spider Gates'. And, when you attempted to do this, it was nearly impossible to get all the gates to line up without any whitespace inbetween (more about that in a minute). It was nice being able to easily match that slope with a set of quadrants in DiVa, but that wouldn't translate into FlowJo, so you were forced to create individual polygons for each of the populations. The slope of the line will depend on the amount of spillover and the spread that ensues. Therefore, it only makes sense that the line you draw to differentiate between single positives and double positives should have a positive slope to it. The positive fraction is always a bit higher than the negative fraction.
One of the features I like best on the DiVa 6 software is the ability to bend quadrant lines to account for the spread that is revealed after you compensate. Our current hourly rates are shown below please note that Comprehensive Cancer Center members receive a lower, subsidized rate for cancer-related research.FINALLY!!! This has been on my list of FlowJo improvements for a long time. Flow Cytometry Shared Resource recharge rates Please contact the Flow Cytometry Shared Resource’s staff to sign up today! Once you are added to the subscription service, you’ll be able to access FlowJo at your convenience provided you have an active internet connection. FlowJo includes tools to analyze flow cytometry data files recorded on any flow cytometer platform, and offers advanced gating, cell cycle, proliferation, multi-color cluster analysis, display and data summary tools. The Flow Cytometry Shared Resource offers investigators low-cost access to the powerful data analysis tools provided by FlowJo, the leading flow cytometry data analysis package.